Coding

Part:BBa_I766101:Design

Designed by: Robert Ovadia   Group: iGEM07_UCSF   (2007-08-09)


mCherry/RFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 364
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 364
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 364
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 364
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Not a standard Biobrick. Cloned using primers containing adaptor regions for combinatorial cloning using type IIs restriction enzymes.


Source

Originally isolated from Discosoma striata.

References